P4210-35D
PIWIL1 (Piwi-like Protein 1, HIWI)
Size
£767.00
- SKU:
- P4210-35D
- Application:
- ICC
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to aa82-290 from human PIWIL1/HIWI expressed in E.coli.
- Clone:
- 12K163
- Format:
- Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
- Extra Details:
- HIWI, also called PIWIL1 is an 861aa, 99kD (predicted) cytoplasmic protein of the PIWI subgroup of the Argonaute family. PIWI domains bind a class of ~29-30 nucleotide RNAs termed piRNAs. PIWIL1 orthologs are expressed with piRNAs in germ cells and are essential for spermatogenesis in mice. CD34+ hematopoietic progenitor cell PIWIL1 is downregulated upon differentiation. When overexpressed in leukemic cell lines, PIWIL1 inhibits growth and induces apoptosis. Human PIWIL1 shares 95% aa sequence identity with mouse and rat PIWIL1. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice