P4205-75B

Pirh2 (p53-induced RING-H2 Protein, Androgen Receptor N-terminal Interacting Protein, ARNIP, CH-rich interacting match with PLAG1, CHIMP, DKFZp586C1620, PRO1996, Ring Finger and CHY Zinc Finger Domain Containing 1, RCHY1, Zinc Finger Protein 363, ZFP363)

Size

£607.00

SKU:: P4205-75B
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant fragment of human Pirh2 expressed in E. coli.
Clone:: 10F610
Format:: Supplied as a liquid, 0.03% sodium azide.
Extra Details:: Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function. Applications: Suitable for use in ELISA, Western Blot. and Immunofluorescence. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunofluorescence: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.