P4205-74Y

Pirh2 (p53-induced RING-H2 Protein, hPirh2, Androgen Receptor N-terminal Interacting Protein, ARNIP, CH-rich Interacting Match with PLAG1, CHIMP, E3 Ubiquitin-protein Ligase Pirh2, Ring Finger and CHY Zinc Finger Domain Containing 1, RCHY1, RING Finger Protein 199, RNF199, Zinc Finger Protein 363, ZNF363)

Size

£581.00

SKU:: P4205-74Y
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues near the C-terminus of human RCHY1 protein isoform1. Species sequence homology: monkey (100%)
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Extra Details:: RING finger and CHY1 zinc finger domain containing protein 1 (RCHY1) is a newly identified RINGH2- type protein-ubiquitin E3 ligase that is expressed as multiple isoforms generated through alternative splicing of mRNA transcripts (1,2). Notably, RCHY1 is a p53-regulated gene and mutiple studies have shown that RCHY1 directly binds p53 to promote its ubiqutin-dependent proteasomal degradation, ultimately leading to repression of p53 growth suppressive transcriptional activity (1,3). RCHY1 is also likely to promote deregulated cell proliferation by targeting the CDK inhibitor, p27/KIP1, for proteasomal degradation (4). In agreement with its growth promoting activity, RCHY1 overexpression hs been linked to cancer of the lung (5,6) and prostate (7). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice