P4186-11A

PI 3-Kinase, p120 gamma, Recombinant, Human, His-Tag (Phosphatidylinositol-PI-3-Kinase)

Size

£1,194.00

SKU:: P4186-11A
Molecular Weight:: 130
Purity:: Purified by Ni2+/NTA-agarose. ~77% by SDS-PAGE and Coomassie blue staining.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 2mM DTT, 10% glycerol.
Extra Details:: N-terminal His6-tagged recombinant full-length human PI 3-kinase (p120g) expressed by baculovirus in Sf21 insect cells. Specific Activity: ~35U/mg, where one unit of PI3 Kinase (p120γ) activity is defined as 1nmol phosphatidylinositol 3,4,5-trisphosphate formed/minute at 22°C with a final ATP concentration of 100uM. MS Tryptic Fingerprint: Confirmed identity as PI 3-Kinase (p120g) with 37% amino acid coverage of the translated native sequence Kinase Assay: 0.05-12.5ng of PI 3-Kinase (p120g) was assayed in a 30 minute reaction. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.