P4079C
Phosphotyrosine (AP)
Size
£512.00
- SKU:
- P4079C
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Phosphotyrosine conjugated to carrier proteins
- Clone:
- PY20
- Format:
- Supplied as a liquid in Tris, pH 8.0, 1mM magnesium chloride, 0.09% sodium azide, 50% glycerol. Labeled with alkaline phosphatase (AP).
- Extra Details:
- Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during normal growth and development and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation is cell surface glycoproteins. Antibodies to phosphotyrosine enable the detection, isolation, and characterization of proteins containing phosphotyrosine. The monoclonal antibody PY20 prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry/Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice