P4078-10

Phosphotyrosine Control, EGF-Stimulated A431 Cell

Size

£881.00

SKU:: P4078-10
Purity:: Cellular protein preparation from A431 cells containing phosphorylated proteins, including the EGF receptor.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a frozen solution in RIPA sample buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin, 1mM Na3VO4, 1mM NaF).
Extra Details:: Applications: Positive control for anti-phosphotyrosine antibodies. Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: Detects the phosphorylated EGF receptor (170kD) and other tyrosine phosphorylated proteins in 20ug of the preparation. Add an equal volume of Laemmli reducing sample buffer to 5-25ul of lysate and boil for 5 min to reduce the preparation. Immunoprecipitation Positive Control: This preparation may also be used for immunoprecipitation with anti-phosphotyrosine agarose. Storage and Stability: Store at -20ºC Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.