P4078-09

Phosphotyrosine (FITC)

Size

£1,179.00

SKU:: P4078-09
Application:: ICC
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Phosphotyramine (KLH coupled)
Clone:: 4G10
Format:: Supplied as a liquid in PBS, pH 7.4. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 5ug/ml. Detects phosphotyrosine containing proteins in EGF-stimulated human A431 cells. Optimal dilutions to be determined by researcher. Hybridoma: FOXY-NY (a NS-1 derivative) crossed with spleen cells from immunized mice. Positive Control: Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice