P4078-01A-BIOTIN
Phosphotyrosine (Biotin)
Size
£929.00
- SKU:
- P4078-01A-BIOTIN
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human phosphotyrosine coupled to carrier protein.
- Clone:
- 5J256
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Extra Details:
- Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 1:2000 Western Blot: 1:2000 Immunohistochemistry: 1:500 Immunocytochemistry: 1:500 Immunoprecipitation: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice