P4077
Phosphothreonine
Size
£780.00
- SKU:
- P4077
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Phosphothreonine conjugated to KLH and phosvitin.
- Format:
- Supplied as a liquid in PBS, pH 7.0, 0.01% sodium azide, 50% glycerol.
- Extra Details:
- Protein phosphorylation is a complex biochemical process that ultimately influences the mechanism by which a cell responds to a particular stimulus. A class of enzymes referred to as protein kinases regulates this process; these enzymes catalyze the transfer of the gama-P of ATP to a recipient protein that acts as a substrate. This post-translational modification is primarily directed onto serine and threonine amino acid residues within a protein. It has been noted that changes in the phosphorylation status of these phosphoproteins can alter the biological function of a protein. Additionally, aberrations in signal transduction cascade have been linked to a number of diseases including cancer, diabetes, heart disease, inflammation and neurological disorders. Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 2ug/ml; Mouse brain lysate. Incubate at RT for 4 hrs. ELISA: 0.5ug/ml (kinase assay). Binding of Ly-pT-Gly-HRP (0.2ug/100ul/well) to an anti-phosphothreonine coated microplate (0.5ug/100ul/well) is inhibited by 95% with the addition of 2-10ug/ml anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1mg/ml. Immunohistochemistry: paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice