P4077-51

Phosphothreonine Proline

Size

£672.00

SKU:: P4077-51
Application:: WB
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Phosphothreonine-proline amide (pT-P-NH2) peptide conjugates (KLH).
Format:: Supplied as a liquid in TBS, 0.05% sodium azide, 50% glycerol.
Extra Details:: Phosphothreonine-Proline, along with similar motifs involving serine/threonine residues preceding proline undergoing phosphorylation, has critical roles in the moderation of various cellular processes, protein function, and kinases through proline-directed phosphorylation. The deregulation of proline-directed phosphorylation is known to be a factor in diseases such as cancer and Alzheimer’s neurodegeneration. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500 detected phosphorylated proteins on 10ug of calyculin treated Jurkat lysates. Optimal dilutions to be determined by the researcher. Positive Control: Calyculin treated Jurkat cell lysate. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.