P4077-14
Phosphothreonine
Size
£355.00
- SKU:
- P4077-14
- Purity:
- Purified by thiophilic adsorption and size exclusion chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic Phosphothreonine containing peptides conjugated to KLH.
- Clone:
- 6D665
- Format:
- Supplied as a lyophilized powder from PBS, 0.09% sodium azide, PEG and sucrose. Reconstitute with 1ml sterile dH2O.
- Extra Details:
- Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 0.05ug/ml Western Blot: 1ug/ml for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween20 based buffer. Do not use milk or casein for blocking. Immunoprecipitation: 1-10ug per 10e6 pervanadate-treated A431 cells. Optimal dilutions to be determined by the researcher. Positive Control Cell Lysate (Included): P4076-22A: Phosphoserine Positive Control. Use 20ul/lane. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice