P4074-42C-ML490
Phospholipase C, gamma 1 (PLCg1) (MaxLight 490)
Size
£1,023.00
- SKU:
- P4074-42C-ML490
- Purity:
- Purified by Protein G chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified soluble phospholipase C (PLC) g-1 from bovine brain.
- Clone:
- 0.T.117 (B-6-4)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Phospholipase C gamma 1 is a tryosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-triphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLC gamma activity is unknown. Applications: Suitable for use in FLISA, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 0.1-0.5ug/ml. Detects PLCg1 in acid precipitated protein from bovine brain, RIPA lysates from A431 cells, and lysates from 3Y3/A31 fibroblasts and L6 myoblasts. Immunoprecipitation: 4ug (bovine brain cytosol). Optimal dilutions to be determined by the researcher. Positive Antigen Control (Included): A0001-50 (Non-stimulated A431 cell lysate). Add 2.5ul of 20mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Use 20ug per lane for minigels. Storage and Stability: May be stored at -20°C. Reconstitute to nominal volume by adding ddH2O and 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice