P4074-42
Phospholipase C, gamma 1 (PLCg1)
Size
£767.00
- SKU:
- P4074-42
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Five individual clones raised against purified soluble phospholipase C (PLC) g-1 from bovine brain.
- Clone:
- B-2–5, B-6- 4, B-20–3, D-7–3, E-9–4.
- Format:
- Supplied as a lyophilized powder from 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. Reconstitute with 40ul dH2O.
- Extra Details:
- Phospholipase C gamma 1 is a tryosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-triphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLC gamma activity is unknown. Applications: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detects PLCg1 in human A431 carcinoma cells as well as rabbit and bovine brain cytosol preparations. Immunoprecipitation: 4ug Immunoprecipitates PLCg1 from non-stimulated A431 cell lysates and from rabbit brain cytosol preparation. Immunocytochemistry: 5-10ug/ml detects PLCg1 on ethanol-acetic acid (95:5) fixed A431 cells. Optimal dilutions to be determined by the researcher. Positive Antigen Control (Included): A0001-50 (Non-stimulated A431 cell lysate). Add 2.5ul of 20mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Use 20ug per lane for minigels. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice