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P4074-40-ML490

Phospholipase C, beta 1 (PLCB1, KIAA0581, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1, PLC-154, Phosphoinositide phospholipase C-beta-1, Phospholipase C-I, Phospholipase C-beta-1) (MaxLight 490)

Size

£1,055.00

SKU:
P4074-40-ML490
Purity:
Purified by Protein G affinity chromatography.
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Mixed monoclonal antibodies raised against purified bovine PLCβ-1
Clone:
0.T.171 (K-32-3, K-92-3)
Format:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Extra Details:
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Applications: Suitable for use in Immunoprecipitation/Western Blot. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation/Western Blot: 5ug immunoprecipitated PLC b-1 from a bovine brain membrane preparation. The immunoprecipitation procedure was followed by an Western Blot using 5ug/ml of P4074-40 and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice