P4074-06D

Phospholipase A2, Secretory, Group IB (PLA2G1B, phospholipase A2, MGC119834, MGC119835, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2 precursor, PLA2, PLA2A, PPLA2)

Size

£642.00

SKU:: P4074-06D
Purity:: Purified by peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to NKAHKNLDTKKYCQS, from the C-Terminus of human Phospholipase A2, Secretory, Group IB (NP_000919.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Group IB secretory phospholipase A2 (sPLA2-IB) mediates cell proliferation, cell migration, hormone release and eicosanoid production via its receptor in peripheral tissues. In the CNS, high-affinity binding sites of sPLA2-IB have been documented. sPLA2-IB induced neuronal cell death in a concentration dependent manner depending on PGD2 metabolites, especially Delta12-PGJ2 that might mediate sPLA2-IB-induced apoptosis. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Peptide ELISA Titer: 1:64,000 Western Blot: 0.01ug/ml; ~17-18kD band observed in Human Pancrease lysates (calcuated MW: 16.4kD according to NP_000919.1). In transfected HEK293 transiently expressing PLAG1B a band of ~18kD is observed. This band is not observed in the non-transfected HEK293. Optimal dilutions to be determined by the researcher. Positive Control: Human Pancreas lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice