P4073

Phosphoglucomutase, beta (PGM)

Size

£914.00

SKU:: P4073
Purity:: Purified from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: beta-Phosphoglucomutase [Lactococcus lacti]
Format:: Supplied as a powder lyophilized from a solution containing PBS, pH 7.2 and 0.01% sodium azide. Reconstitute with 100ul ddH20.
Extra Details:: Phosphoglucomutase (PGM) interconverts the 1- and 6-phosphate isomers of alpha-D-glucose. Although it is a reversible reaction, the formation of glucose-6-phosphate is markedly favored. PGM will not convert beta-D-glucose-1-phosphate or beta-D-glucose-6-phosphate. Applications: Suitable for ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:15,000 - 1:60,000. Tested against 1ug of beta-Phosphoglucomutase [Lactococcus lacti] in a standard sandwich ELISA using peroxidase conjugated rabbit anti-goat IgG [H&L] and ABTS as as substrate (incubation: 30 minutes at RT). Western Blot: 1:500 - 1:3,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.