P4073-23A
Phosphoinositide 3 Kinase, p55 gamma (PI-3 Kinase p55 gamma, Phosphoinositide-3-kinase Regulatory Subunit 3 (p55, gamma), PIK3R3)
Size
£806.00
- SKU:
- P4073-23A
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Lys251-Gly378 from human PI 3-Kinase p55g, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- p55g (Protein of 55kD, gamma subunit; also PI 3-Kinase regulatory subunit gamma and p55 PIK) is a 55-57kD member of the PI 3K p85 family of regulatory subunits. It is widely expressed and serves as a recruitment subunit for the p110 catalytic subunit of PI 3-Kinase. p55g/p55 PIK should not be confused with the 55kD alpha splice variant of the p85 alpha gene (75% aa identity). Human p55g is 461aa in length and contains a unique 34aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa35-44) and two SH2 domains that bind the p110 catalytic subunit (aa65-160 and 358-452). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml. Validated using lysates of SH-SY5Y human neuroblastoma cell line, C2C12 mouse myoblast cell line, and rat-2 rat embryonic fibroblast cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice