P4073-18M

PGAM1, PGAM2, PGAM4 (Phosphoglycerate Mutase 1, BPG-dependent PGAM 1, Phosphoglycerate Mutase Isozyme B, PGAM-B, PGAMA, CDABP0006/Phosphoglycerate Mutase 2, BPG-dependent PGAM 2, PGAM-M, Muscle-specific Phosphoglycerate Mutase, Phosphoglycerate Mutase Isozyme M, PGAMM/Probable Phosphoglycerate Mutase 4, PGAM3)

Size

£642.00

SKU:: P4073-18M
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-KAMEAVAAQGKAKK, from human PGAM1/PGAM2/PGAM4, at C-terminal (NP_002620.1, NP_000281.2, NP_001025062.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: PGAM is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM in mammals, a muscle and brain isoform. PGAM has also been linked to non-spherocytic anemia. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:128,000 Western Blot: 0.01-0.03ug/ml, observed in human liver and brain lysates on ~28kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.