P4072-11J

PDE11A (Dual 3',5'-cyclic-AMP and -GMP Phosphodiesterase 11A, cAMP and cGMP Phosphodiesterase 11A)

Size

£642.00

SKU:: P4072-11J
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-NSFKESMEKSSYSD, from human PDE11A, at the internal region of the protein (NP_058649.3, NP_001070665.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: PDE11A is a 100kD cyclic nucleotide phosphodiesterase, which catalyzes the hydrolysis of cAMP and cGMP. PDE11A is thought to be involved in multiple physiological processes in various organs. PDE11A is calmodulin dependent and exists as four splice variants (PDE11A1-PDE11A4). Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Western Blot: 0.5-1.5ug/ml, observed in human skeletal muscle and prostate lysates on ~60-65kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.