P4071-22B
Phosphatase, Alkaline (ALPL) (PE)
Size
£741.00
- SKU:
- P4071-22B
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human liver, bone and kidney-derived Alkaline Phosphatase.
- Clone:
- 7H259
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Alkaline phosphatase (ALP) is a cell surface enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. Most mammals exhibit four isozymes of ALP with selective expression in distinct tissues; placenta, placenta-like, intestine, and tissue non-specific. The liver/bone/kidney isozyme, also called tissue non-specific alkaline phosphatase, was originally cloned from an osteosarcoma cell line screened with anti-liver ALP antiserum. Antibodies to ALP have been used in lineage studies to characterize the development of osteoclasts and adipocytes. In addition to its expression on the cell surface, neutrophil alkaline phosphatase is found within the secretory granules of neutrophils and is released into the serum. Hypophosphatasia, a genetic metabolic disorder resulting from a deficiency in serum and bone alkaline phosphatase activity, manifests itself in patients with severe skeletal defects. Neutrophil alkaline phosphatase is often used diagnostically in various pathologies including myeloid cancers, Down’s syndrome and Turner syndrome. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells in BG01V human cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice