P4071-22A
Phosphatase, Alkaline
Size
£812.00
- SKU:
- P4071-22A
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Leu18-Ser502 from human ALPL, expressed in E. coli.
- Clone:
- 7H104
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Alkaline phosphatase, liver/bone/kidney encoded by the ALPL gene, also known as tissue nonspecific alkaline phosphatase (TNAP), is related to, but distinct from, intestinal/ALPI, placental/ALPP and placental-like/ALPPL2 alkaline phosphatases. ALPL is highly expressed in undifferentiated human embryonic stem cells, embryonic carcinoma cells, and embryonic germ cells. Mutations in this enzyme have been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. The amino acid sequence of human ALPL is 90%, 89% and 75% identical to that of rat/mouse, canine and chicken. Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-2ug/ml. The detection limit is ~10 ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~2ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice