P4071-16N
Phosphatase, Alkaline, Placental (PLAP, Alkaline Phosphatase Placental, Alkaline Phosphatase Placental Type, ALP, ALPP, Glycerophosphatase, PLAP-1, Regan Isozyme)
Size
£832.00
- SKU:
- P4071-16N
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ile23-Asp506 from human ALPP expressed in NS0 cell line.
- Clone:
- 12K161
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Four distinct genes encode alkaline phosphatases in humans. The ALPP gene encodes the 67kD placental isozyme, also known as PLAP. In comparison, ALPI, ALPL, and ALPPL2 encode intestinal, liver/bone/kidney, and placental-like or germ cell APs, respectively. The placental alkaline phosphatase is a glycosyl phosphatidylinositol (GPI) anchored protein that is expressed on the cell surface as a homodimer. Human APs are ectopically expressed in several cancers, and their serum levels are useful tumor markers. Mature human Alkaline Phosphatase/ALPP shares 74% aa sequence homology with the mouse and rat enzymes. Applications: Suitable for use in Western Blot, Direct ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using JAR human choriocarcinoma cell line Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human Alkaline Phosphatase/ALPP Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice