P4071-16D

Phosphatase, Alkaline, Placental (Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, ALP, FLJ61142, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1)

Size

£812.00

SKU:: P4071-16D
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: HEp-2 cells expressing Placental Alkaline Phosphatase (PLAP)
Clone:: 0.N.515
Format:: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and Azide. See P4214-10X.
Extra Details:: Antibody to PLAP is useful in the identification of germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA). Applications: Suitable for use in ELISA and Immunohistochemistry. For coating ELISA use P4214-10X. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Formalin/paraffin): 1:200 for 20 minutes at RT. Staining of formalin-fixed tissues requires digestion with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C. Optimal dilutions to be determined by the researcher. Cellular Localization: Cell membrane. Positive Control: Placenta Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.