P4071-04E
Phosphatase, Alkaline, Bone (Akp2, ALPI, ALPL)
Size
£691.00
- SKU:
- P4071-04E
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Alkaline phosphatase from human bone.
- Clone:
- 10B141 (BGN/03/661)
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP). Although the exact biochemical function of BAP is uncertain, measurement of the serum levels of BAP can be used as a biochemical indicator of bone turnover. Conditions which present with a decrease in the level of BAP (hypophosphatasemia) include the inherited bone-deforming disorder hypophosphatasia and osteoporosis, while an increase in BAP (hyperphosphatasemia) is associated with Paget’s disease, bone fractures and osteosarcomas. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: Detects a band at ~70kD Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice