P4071-03B

Phosphatase, Alkaline, Calf Intestine (1,500-2,500u/mg)

Size

£143.00

SKU:: P4071-03B
Purity:: ≥95%
Storage Conditions:: -20°C
Supplier:: United States Biological
CAS Number:: 9001-78-9
Format:: Supplied as a lyophilized powder.
Extra Details:: Alkaline Phosphatase is used to dephosphorylate dsDNA and RNA in molecular cloning and DNA sequencing reactions. This is a cost effective conjugation grade used in antibody preparation supplied as a lyophilized powder. Activity: 1,500-2,500 units/mg protein Unit Definition: The amount of enzyme which liberates 1umole of p-nitrophenol from p-nitrophenyl phosphate per minute at 37°C, pH 9.8 in diethanolamine buffer. Quality Control: SDS-PAGE No contaminating RNase, DNase or Proteases detected Activities: PDE ≤0.0004% Adenosine Deaminase ≤0.004% A-5-MP Deaminase ≤0.004% Recommended Dilution: Optimal dilutions to be determined by the researcher. Country of Origin: Prepared from US origin starting material and collected in USDA approved facilities. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with distilled water or sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.