P4071-03

Phosphatase, Alkaline, Calf Intestine, Solution (~3000u/mg)

Size

£147.00

SKU:: P4071-03
Storage Conditions:: 4°C
Supplier:: United States Biological
CAS Number:: 9001-78-9
Extra Details:: Alkaline Phosphatase is used to dephosphorylate dsDNA and RNA in molecular cloning and DNA sequencing reactions. Source: Calf Intestine Prepared from US origin starting material and collected in USDA approved facilities Protein Concentration: 1mg/ml Assay Method: Based on the method of Garen and Levinthal whereby the reaction velocity is derived by measuring the absorbance increase at 410nm after hydrolysis of p-nitrophenyl phosphate. Form: Supplied as a liquid in deionized water. Activity: ~3000 units/mg protein (glycine units). 1 glycine unit = 2-3 DEA units Unit Definition: One unit of enzyme hydrolyzes 1umole of p-nitrophenyl phosphate per minute at 37°C, pH 9.8 in the presence of Mg and Zn. Quality Control: SDS-PAGE No contaminating RNase, DNase or Proteases detected Storage and Stability: Stable for 1 year at 2-8ºC