P4070-88F

PHLPP2 (PH Domain Leucine-rich Repeat-containing Protein Phosphatase 2, KIAA0931, PH Domain Leucine-rich Repeat-containing Protein Phosphatase-like, PHLPP-like, PHLPPL)

Size

£642.00

SKU:: P4070-88F
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-PHEEDRTEPPEEFD, from human PHLPPL, at C-terminal (NP_055835.2).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: PHLPP2, a protein phosphatase and negative regulator, which mediates the inactivation of Akt and protein kinase C (PKC) activity, through the direct dephosphorylation of key regulatory sites: AKT1 (Ser473), PRKCA (Ser657) and PRKCB isoform beta-II (Ser660). PHLPP2, and its alternative isoform PHLPP1, have been implicated as important suppressors of tumor cell growth, being linked with breast, ovarian and colon cancer. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.1-0.3ug/ml, observed in human brain (amygdala, hippocampus, substantia nigra) lysates on ~140kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.