PHD3 (PH3, Prolyl Hydroxylase Domain-containing 3, HIF2PH3, HIF-Prolyl hydroxylase 3, EGLN3, egl Nine Homolog 3, SM-20, FLJ21620)

Size

£767.00

SKU:: P3375-07
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 16aa peptide from the C-terminal region of human PHD3/EGLN3.
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Extra Details:: Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline. PHD3 (human 333-aa, chromosome 14; also known as EGL9, C. ELEGANS, HOMOLOG OF, 3, EGLN3, PROLYL HYDROXYLASE DOMAIN-CONTAINING PROTEIN 3;PHD3, HIF-PROLYL HYDROXYLASE 3; HIFPH3) Widely expressed at low levels. Expressed at higher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta. It is activated under hypoxia. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:100,000; Using 50-100ng of control peptide/well. See P3375-07A. Western Blot: 1-5ug/ml using ECL technique. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice