PHD1 (PH1, prolyl hydroxylase domain-containing protein 1, HIFPH1, HIF-prolyl hydroxylase 1, EGLN2, EGL nine homolog 2, EIT6, estrogen-induced tag 6, DKFZp434E026)

Size

£754.00

SKU:: P3373-05
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A synthetic 22aa peptide of the C-terminus human PHD1/EGLN2.
Format:: Supplied as a liquid in PBS, pH 7.2.
Extra Details:: Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline. PHD1 (human 407-aa, chromosome 19; also known as Egl nine homolog 2, EGLN2 (Hypoxia-inducible factor prolyl hydroxylase 1) (HIF-prolyl hydroxylase 1) (HIF-PH1) (HPH-3) (Prolyl hydroxylase domain-containing protein 1) (PHD1) (Estrogen-induced tag 6) is a hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. PHD1 is expressed abundantly in all tissues with highest expression in testis. It is expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:100,000; Using 50-100ng of P3373-05A control peptide/well. Western Blot: 1-5ug/ml using ECL technique. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice