PHAP III, CT (LANPL, LANP-L, Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member E, LANP-like Protein)

Size

£655.00

SKU:: P3368-04
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to amino acids close to C-terminus of human PHAP III. Epitope: within amino acids 200-250
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Extra Details:: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml, band at ~35kD Immunohistochemistry (paraffin): 2ug/ml Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher. Blocking Peptide: P3368-04A Positive Control: A549 or HepG2 cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice