P3363-60

PGCP (Plasma Glutamate Carboxypeptidase)

Size

£863.00

SKU:: P3363-60
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Mouse myeloma cell line NS0-derived recombinant mouse PGCP, Lys19-Ser470.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: PGCP (plasma glutamate carboxypeptidase) is a secreted member of the M28 family of metallopeptidases. Mouse PGCP is 470 amino acids (aa) in length. It apparently contains one annexin-like region (aa 19-68) plus a peptidase M28 catalytic domain (aa 281-443). It appears to be synthesized by the liver. Based on human, mouse PGCP will show a 97 aa substitution for the C-terminal 26 amino acids. Over aa's 19-470, mouse PGCP shares 89% and 94% aa identity with human and rat PGCP, respectively. Applications: Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.