P3352-34
Peroxiredoxin 5 (PRDX5, ACR1, AOEB116, B116, PLP, PMP20, PRDX6, PRXV, SBBI10)
Size
£863.00
- SKU:
- P3352-34
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Purified, E. coli-derived, recombinant mouse Peroxiredoxin 5 (rmPrx-5; aa 1-210; Accession# P99029)
- Format:
- Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 100ul sterile PBS.
- Extra Details:
- Peroxiredoxin 5 (Prx-5; also known as AOEB166 and thioredoxin reductase) is a 22kD, widely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins. The mouse precursor molecule is 210 amino acids (aa) in length and contains an N-terminal 48 aa mitochondrial targeting (signal) sequence and a 162 aa mature enzyme that shows an NES between aa 54-63, and a peroxisome targeting motif at aa 208-210. There are two catalytic cysteines, one at Cys96, and another at Cys200 of the precursor. Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes. It is known to reduce H 2O2 and alkyl hydroperoxides. Two potential splice forms are reported. One is 17kD in size and shows an alternative start site at Met49, while a second shows a 19 aa substitution for aa 83-102. Mouse Prx-5 is 78% and 90% aa identical to human and rat Prx-5, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice