P3352-25A
Peroxiredoxin 4 (Peroxiredoxin-4, PRDX4, PRX-4, Peroxiredoxin IV, Prx-IV, Antioxidant Enzyme AOE372, AOE37-2, Thioredoxin Peroxidase AO372, Thioredoxin-dependent Peroxide Reductase A0372)
Size
£863.00
- SKU:
- P3352-25A
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to human Peroxiredoxin 4 (aa1-271; Accession# Q13162), expressed in E. coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Peroxiredoxin 4 (Prx-IV; also known as PRDX4 and thioredoxin peroxidase AOE372) is a 24-26kD, widely expressed cytoplasmic antioxidant enzyme that belongs to the typical 2-Cys class of the TSA/ahpC family of peroxiredoxins. It exists as either a homodimer, or heterodimer with PRDX1. PRDX4 reportedly counteracts p53-mediated generation of ROS, and regulates NFκB via IκB-α. Human PRDX4 is 271 amino acids (aa) in length and contains an N-terminal poly-Leu region (aa 20-30) and a 159 thioredoxin domain (aa 79-237). There is one catalytic cysteine at Cys124. Over amino acids 1-271, human PRDX4 shares 89% aa identity with mouse and rat PRDX4. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice