P3351-95C
Peroxiredoxin 1 (PRDX1)
Size
£895.00
- SKU:
- P3351-95C
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- E. coli-derived recombinant protein corresponding to Met1-Lys199 from hhuman Peroxiredoxin 1. Species sequence homology: mouse (96%).
- Clone:
- 9j9
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Human Peroxiredoxin1 (Prx-1, also known as Thioredoxin peroxidase 2) is a 22kD antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The molecule is 199aa in length and has two catalytic cysteines, one at Cys52 and a second at Cys173. Prx-1 is an obligate homodimer. In its inactive state, Prx-1 is apparently noncovalently associated. Upon peroxide binding to Cys52 of subunit 1, the Cys173 of subunit 2 interacts with Cys52 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys52 and Cys173. Subsequent reduction restores the subunits to the basal state. There are apparently two additional isoforms; one shows a premature truncation after aa171, while the second shows a deletion of aa21-121. Human Prx-1 is 96% aa identical to mouse Prx-1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice