P3350-27

Peroxidase, Horseradish (HRP) (Not for Export EU)

Size

£626.00

SKU:: P3350-27
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Peroxidase (Horseradish).
Format:: Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 2ml sterile ddH20
Extra Details:: Horseradish Peroxidase (HRP) is a hemoprotein catalyzing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome C and the leuko form of many dyes. Horseradish Peroxidase has been found well suited for the preparation of enzyme conjugated antibodies, due in part to its ability to yield chromogenic products and to its relatively good stability characteristics. Applications: Suitable for use in ELISA, Dot Blot, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:10,000-1:40,000 Western Blot: 1:1000-1:4000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.