P3342-01
Period Clock Protein 1 (dPER) (Drosophila)
Size
£754.00
- SKU:
- P3342-01
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 21aa within the C-terminus of Drosophilla Period Clock Protein 1, conjugated to KLH.
- Format:
- Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
- Extra Details:
- Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm. Human and mouse genes encoding a basic helix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported. The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively. Homologues of mPer1 designated Per 2 (1257aa) and Per3 (1113aa) have also been cloned. Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes. It has been suggested that mPer regulates neuronal activity in the SCN. Using genetic approach, a single mutation of A to T in the Clock gene affects circadian rhythmicity in mice. Clock has been mapped to chromosome 5. Mouse Clock encodes a transcription factor, a single polypeptide chain of 855aa (predicted calculated MW~97kD; pI 6.52; hClock, 846aa). Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, lung, and kidney). Although Clock is constitutively expressed (not rhythmic) in the SCN, it may still be an important component of circadian machinery. Applications: Suitable for use in ELISA. Other potential application, though not tested is Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000. Using 50-100ng of P3342-01A control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice