P3340-10
Perforin (Cytolysin)
Size
£505.00
- SKU:
- P3340-10
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Full length recombinant protein corresponding to rat perforin, expressed in E.coli.
- Format:
- Supplied as a lyophilized powder, 0.09% sodium azide. Reconstitute with PBS.
- Extra Details:
- Perforin, originally cloned from mouse cytotoxic T-lymphocyte (CTL), is a 555-amino acid protein with a 21-amino acid signal peptide and is a potent cytolytic pore-forming protein. It is a specific marker of functionally active cytotoxic T-lymphocytes (CTLs) and natural killer (NK) cells. Fas ligand and perforin are the two key effector mechanisms in T cell-mediated cytotoxicity. These molecules mediate cytolysis of target cells by membrane damage and apoptosis, an important mechanism in the immune defense against tumors and virus infection. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice