P3272-06

Pepsinogen I

Size

£709.00

SKU:: P3272-06
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human pepsinogen I (native).
Clone:: 8.BG.1 (99/12)
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Pepsinogen I, a zymogen, or proenzyme secreted by Chief cells in the stomach, is cleaved to form pepsin both in an autocatalytic fashion and by pepsin itself. In humans there are two related forms of pepsin, Pepsinogen I, also known as Pepsinogen A, and Pepsinogen II, also known as Pepsinogen B or progastricsin. Applications: Suitable for use in ELISA, Immunohistochemistry and RIA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Paraffin sections. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher. Affinity Constant: 4x10e10L/mol Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.