P3179-10A
Penicillinase, E. clocae (Biotin)
Size
£889.00
- SKU:
- P3179-10A
- Purity:
- Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Penicillinase [Enterobacter cloacae]
- Format:
- Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, before the addition of glycerol to 40%. Labeled with biotin.
- Extra Details:
- Penicillinase is a specific type of β-lactamase, showing specificity for penicillins, again by hydrolysing the beta-lactam ring. Molecular weights of the various penicillinases tend to cluster near 50kD. Penicillinase was the first β-lactamase to be identified: It was first isolated by Abraham and Chain in 1940 from Gram-negative E. coli even before penicillin entered clinical use,[2] but penicillinase production quickly spread to bacteria that previously did not produce it or produced it only rarely. Penicillinase-resistant beta-lactams such as methicillin were developed, but there is now widespread resistance to even these. Penicillinases are classified as Group 2 along with cephalosporinases, both are inhibited by clavulanic acid, corresponding to the molecular classes A and D reflecting the original TEM and SHV genes. Due to the increasing number of TEM- and SHV-derived β-lactamases, Penicillinases are further divided into the subclass 2a. Applications: Suitable for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence as well as other antibody based assays using streptavidin or avidin conjugates. Recommended Dilution: ELISA: 1:2000 to 1:10,000. 1ug of Penicillinase was used in a standard capture ELISA with ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice