P3123-07
PDK1 Docking Motif, Phosphorylated (Ser/Thr) (PDPK1, PI–P3 Dependent Kinase, 3a Phosphoinositide-dependent Protein Kinase 1)
Size
£691.00
- SKU:
- P3123-07
- Purity:
- Ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptides corresponding to the PDK1 docking motif, followed by extensive screening and characterization of hybridoma clones. Motif: (F/K)XX(F/Y)(S*/T*)(F/Y).
- Clone:
- 3i23(18A2)
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
- Extra Details:
- A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases (1), but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3 (2). These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Our phospho-motif specific antibodies provide new tools for probing the complexity of phosphoregulatory pathways. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine in a hydrophobic motif (3). For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site preceded by phenylalanine at -4 and -1 and followed by tyrosine at +1 (4). RSK2, p70 S6 K and certain PKC isoforms also contain a similar consensus phosphorylation motif. Phosphorylation of these motifs is required for binding to 3-phosphoinositide-dependent kinase 1 (PDK1) (5-7). This antibody is a powerful tool for the characterization of phosphorylated PDK1 docking motifs and the identification of new proteins with PDK1 docking motifs. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:20 ELISA (Peptide): 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice