P3122-05G
P4HB (Protein Disulfide-isomerase, PDI, Prolyl 4-hydroxylase Subunit beta, Cellular Thyroid Hormone-binding Protein, p55, PO4DB, PDIA1, PDI, ERBA2L)
Size
£825.00
- SKU:
- P3122-05G
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to aa18-505 from human Protein Disulfide Isomerase/P4HB expressed in CHO cells (P07237).
- Clone:
- 11n150
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- P4HB is a 60kD member of the protein disulfide isomerase family. As an intracellular homodimer, it forms a tetrameric complex with P4H alpha chains to form an active prolyl 4-hydrolyase. This catalyses the hydroxylation of proline in collagen. On the cell surface, it reduces disulfide bonds in HIV that allow the virus to fuse with CXCR4 and enter susceptible cells. Mature human P4HB is 491aa in length. It contains two TRX domains aa25-134 and 368-475 plus an ER retention sequence aa505-508. There is one potential isoform that shows an 11aa substitution for the first 162aa. Over aa18-505, human P4HB shares 94% aa identity with mouse P4HB. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunoprecipitation: 25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice