PCOLE1 (Procollagen C-Proteinase Enhancer-1, PCPE-1, Type I Procollagen C-terminal Enhancer Protein-1)

Size

£856.00

SKU:: P3116-01
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Gln25-Ala468 (predicted) from mouse PCOLE1, expressed in NS0 cells.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: PCOLE1 (PCPE-1) is a secreted 55kD enzyme adaptor that enhances the activity of the BMP-1/tolloid family of metalloproteinases (also known as procollagen C-proteinases) on fibrillar procollagens. PCOLE1 binds to the C-propeptide region and also elsewhere in the procollagen molecule to specifically stimulate BMP-1/tolloid processing of procollagen I, II, and III. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with Recombinant Mouse PCPE‑1 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.