P3115-12A-ML650
PCNA (Proliferating Cell Nuclear Antigen) (MaxLight 650)
Size
£793.00
- SKU:
- P3115-12A-ML650
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rat Proliferating Cell Nuclear Antigen-Protein A expression vector pR1T2T.
- Clone:
- 3H2827 (PC10)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Proliferating cell nuclear antigen (PCNA), formerly known as cyclin, is a 36kD intranuclear acidic protein closely associated with proliferating cells. PCNA was originally identified in patients with systemic lupus erythematosus. It represents a component of DNA polymerase-b. The presence of PCNA is closely associated with the cell cycle. PCNA is expressed in high concentration in the late G1-phase before the DNA synthesis, and it reaches a maximum concentration in the S-phase. The level of PCNA declines during the G2 and M phases. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:50-1:100 (Paraffin). Requires membrane permeabilization. Does not require protein digestion pre-treatment or antigen retrieval of paraffin sections. Immunohistochemistry: Frozen sections Flow Cytometry: 1:100 Use 10ul to label 10e6 cells in 100ul. Requires membrane permeabilization. Western Blot: 1:1000 Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Recommended Positive Control Tissue: Tonsil Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice