P3115-12A-BIOTIN
PCNA (Proliferating Cell Nuclear Antigen) (Biotin)
Size
£793.00
- SKU:
- P3115-12A-BIOTIN
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rat Proliferating Cell Nuclear Antigen-Protein A expression vector pR1T2T.
- Clone:
- 3H2827 (PC10)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- Proliferating cell nuclear antigen (PCNA), formerly known as cyclin, is a 36kD intranuclear acidic protein closely associated with proliferating cells. PCNA was originally identified in patients with systemic lupus erythematosus. It represents a component of DNA polymerase-b. The presence of PCNA is closely associated with the cell cycle. PCNA is expressed in high concentration in the late G1-phase before the DNA synthesis, and it reaches a maximum concentration in the S-phase. The level of PCNA declines during the G2 and M phases. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:50-1:100 (Paraffin). Requires membrane permeabilization. Does not require protein digestion pre-treatment or antigen retrieval of paraffin sections. Immunohistochemistry: Frozen sections Flow Cytometry: 1:100 Use 10ul to label 10e6 cells in 100ul. Requires membrane permeabilization. Western Blot: 1:1000 Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Recommended Positive Control Tissue: Tonsil Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice