P3115-08B-APC
PCNA (Proliferating Cell Nuclear Antigen) (APC)
Size
£1,062.00
- SKU:
- P3115-08B-APC
- Purity:
- Purified by ion-exchange chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rat PCNA made in the protein A vector pR1T2T.
- Clone:
- 2Q2Q19799 (PC10)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Extra Details:
- Proliferating cell nuclear antigen (PCNA) is a 36kD nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure. Applications: Suitable for use in Western Blot, Flow Cytometry, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1-1.0ug/ml Flow Cytometry: 1:100 Immunohistochemistry: (Formalin fixed paraffin embedded tissues): 5-50ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice