P3115-08

PCNA (Proliferating Cell Nuclear Antigen)

Size

£722.00

SKU:: P3115-08
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant full-length protein corresponding to rat PCNA.
Clone:: 2Q197 (PC10)
Format:: Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 10mM sodium azide.
Extra Details:: Proliferating cell nuclear antigen (PCNA) is a 36 kD molecule. PCNA was first identified as the antigen for a subpopulation of autoantibodies in patients with systemic lupus erythematosus It has since been determined that PCNA serves as a co-factor for DNA polymerase delta in S-phase as well as during DNA synthesis associated with mechanisms involved in DNA damage repair. The temporal specificity of PCNA expression makes it an ideal marker for cell proliferation. PCNA begins to accumulate during the G1 phase of the cell cycle, is most abundant during the S phase, and declines during the G2/M phase. Since the half-life of PCNA exceeds 20 hours, it may be possible to detect the protein in non-cycling cells. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and Indirect Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:250-1:1000, Nuclear extracts are preferred over whole cell protein extracts. Detects a polypeptide at 36kD with an isoelectric point of 4.8. Immunohistochemistry (paraffin embedded tissue sections only): 1:20-1:200, may be used on formalin (buffered or unbuffered), methacarn and Bouin’s reagent. The time of fixation can markedly affect the intensity of PCNA immunoreactivity. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air drying overnight on poly-L-lysine coated slides is recommended. Incubate for 1 hour at 25ºC using standard ABC. Optimal dilutions to be determined by the researcher. Positive Control: Tonsil or reactive lymph node Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice