P3115-06A

PCNA (Proliferating Cell Nuclear Antigen)

Size

£668.00

SKU:: P3115-06A
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: 6D646 (IPO-38)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Extra Details:: The proliferating cell nuclear antigen (PCNA) becomes detectable in the G1 phase of the cell cycle and reaches a maximum level of expression during mitosis. PCNA is highly conserved across species and is a critical part of the DNA polymerase delta holoenzyme (1,2,3). The level of PCNA expression depends on the proliferative potential of the cell examined. This monoclonal antibody stains proliferating cells in a wide range of normal tissues, as well as in tissues undergoing pathological processes and after mitogenic and allogeneic stimulations. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Immunohistochemistry: Frozen and paraffin-embedded sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.