P3115-05V
PCNA (Proliferating Cell Nuclear Antigen, Cyclin)
Size
£338.00
- SKU:
- P3115-05V
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to rat PCNA.
- Clone:
- PC10
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- This monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase d auxiliary protein. PCNA is a 36kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase d processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤0.125ug/10e5 cells in 100ul Immunohistochemistry: Acetone-fixed frozen sections Immunohistochemistry: Formalin fixed paraffin embedded sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice