P3114

PCAF, Active, Recombinant, aa352-832 (p300/CBP-associated factor, CREBBP-associated factor, CAF, P/CAF)

Size

£1,298.00

SKU:: P3114
Molecular Weight:: 79
Purity:: ≥30%
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid 50mM Tris-HCl, pH 8.0, 150mM sodium chloride, 25mM glutathione, 50% glycerol.
Extra Details:: A direct link exists between hyperacetylation of chromatin and transcriptional activation. Experiments have established that PCAF possesses intrinsic histone acetylase activity. PCAF primarily acetylates lysine 14 of H3. It acetylates lysine 8 of H4 less efficiently. PCAF also acetylates p53 in response to DNA damage. The distinct patterns of acetylation by PCAF may contribute to transcriptional regulation of important genes. Source: Recombinant GST fusion protein corresponding to aa352-832, expressed in E. coli. Quality Control: HAT Assay: 500ng of PCAF was used to acetylate histones in vitro with tracer levels of [3H]AcetylCoA as the acetyl donor. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.