Pax 2, phosphorylated (Paired Box Gene 2, Paired Box Homeotic Gene 2, Paired Box Protein 2, Paired Box Protein Pax-2, Paired Box Protein Pax2, Pax-2) (HRP)

Size

£934.00

SKU:: P3113-92F-HRP
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2). Species Sequence Homology: mouse, chicken, zebraﬁsh and frog; 100%.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
Extra Details:: Pax2 is a transcription factor encoded by a gene that is required for the embryonic development of a variety of mammalian tissues, including the kidney, optic cup, and inner ear. Structurally, the Pax2/5/8 protein subfamily is characterized by an N-terminal paired domain, a conserved octapeptide sequence, and a paired-paired type homeobox. The N-terminal paired domain of the Pax2/5/8 proteins binds DNA, while the C-terminal region is required for activation of target genes. In human mammary tissue, Pax2 expression has been identified in sub-populations of mammary ductal cells, and also in > 50% of human breast tumors surveyed (n=38). Mouse studies have demonstrated co-localization of Pax2 and WT1 (Wilms’ tumor suppressor protein) in the nuclei of normal and cancerous breast cells, indicating a role for Pax2 in the regulation of the response to progesterone in the mature mammary gland. Pax2 is a phosphorylation target of JNK, and has been observed to co-imunoprecipitate with JIP1. Phosphorylation of Pax2 enhances its ability to transactivate reporter genes. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml; Western Blot analysis of JNK-stimulated (phosphorylated) fusion proteins identiﬁes a single band at ~50kD. In Western Blot analysis of HEK293 cells, a thick band between ~42-45kD is observed, which may represent different human Pax2 isoforms and/or Pax8. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice